The following is an excerpt from Methods in Plant Histology, a science book that was published in the early 1900s. The book contains various kinds of research techniques and methodology regarding plant histology, or the study of the tissue structure of plants. Some of the information and terminology in the excerpt may be outdated.
The hay infusion is a time-honoured method for securing bacteria for study. Pour hot water on a handful of hay, and filter the fluid through blotting paper. Place the fluid in a glass dish, and cover with a piece of glass to keep out the dust. When the fluid begins to appear turbid, bacteria will be abundant. The active movements are easily observed in a mount from the turbid water. As the bacteria pass into the resting condition, they form a scum on the surface of the water. Usually, the first to appear is a somewhat rod-shaped form, the Bacterium term of the older texts. Spirillum and Coccus forms often appear later.
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